Trimming of damaged 3′ overhangs of DNA double-strand breaks by the Metnase and Artemis endonucleases
S Mohapatra, SM Yannone, SH Lee, RA Hromas… - DNA repair, 2013 - Elsevier
Both Metnase and Artemis possess endonuclease activities that trim 3′ overhangs of
duplex DNA. To assess the potential of these enzymes for facilitating resolution of damaged
ends during double-strand break rejoining, substrates bearing a variety of normal and
structurally modified 3′ overhangs were constructed, and treated either with Metnase or
with Artemis plus DNA-dependent protein kinase (DNA-PK). Unlike Artemis, which trims long
overhangs to 4–5 bases, cleavage by Metnase was more evenly distributed over the length …
duplex DNA. To assess the potential of these enzymes for facilitating resolution of damaged
ends during double-strand break rejoining, substrates bearing a variety of normal and
structurally modified 3′ overhangs were constructed, and treated either with Metnase or
with Artemis plus DNA-dependent protein kinase (DNA-PK). Unlike Artemis, which trims long
overhangs to 4–5 bases, cleavage by Metnase was more evenly distributed over the length …
